Fig. 3. Ethanol induces apoptosis signaling in glioblastoma cell lines. (A) Expression levels of mRNA transcripts related with apoptosis were determined by RT-PCR analysis in glioblastoma cell lines (U87MG and U373MG). RNA extraction was performed after 24 h of ethanol exposure GAPDH was used as loading control. (B) Expression levels of proteins associated with apoptosis were measured by Western Blot Analysis in glioblastoma cell lines (U87MG and U373MG). Protein extraction was carried out after 24 h of ethanol exposure. B-actin was used as loading control.
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