Fig. 4. Ethanol induces apoptosis signaling in primary glioblastoma cells. (A) Expression levels of mRNA transcripts involved in apoptosis were measured by RT-PCR analysis in primary glioblastoma cells (GBL-13 and GBL-15). RNA extraction was carried out after 24 h of ethanol exposure. GAPDH was used as loading control. (B) Expression levels of proteins associated with apoptosis were checked by Western Blot Analysis in primary glioblastoma cells (GBL-13 and GBL-15). Protein extraction was performed after 24 h of ethanol exposure. B-actin was used as loading control.
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