Fig. 2. miR-326, miR-330, and miR-3099 expression are increased during differentiation of NSCs, and are reduced by PINK1 deficiency. NSCs were differentiated by growth factor withdrawal (A~C) or treatment with 1 ng/ml CNTF (D~F). (A, D) Expression of markers of neurons (MAP2 and TUJ-1), astrocytes (GFAP), and oligodendrocytes (CNPase) was analyzed 2 and 5 days after NSC differentiation (A) and 4 days after treatment with the indicated amount of CNTF (D). (B, E) miR-326, miR-330, and miR-3099 expression were measured by Q-PCR during WT NSC differentiation. (C, F) miR-326, miR-330, and miR-3099 expression were measured in WT and PINK1-KO NSC at differentiation day 3.
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