Fig. 3. Effects of inhibitors and mimics of miR-326, miR-330, and miR-3099 on GFAP expression. At differentiation day 1, WT NSCs (A, C) or PINK1-KO NSCs (B, D) were transfected with miRNA inhibitors (10 nM) targeting miR-326, miR-330, and miR-3099 (A, C) or miRNA mimics (10 nM) of miR-326, miR-330, and miR-3099 (B, D). WT NSCs were also treated with a mixture of the three miRNA inhibitors (10 nM each) or 30 nM control miRNA. RNU_6 was used as a control. Protein levels of GFAP and TUJ-1 were analyzed by Western blot at differentiation day 5 (A and B, upper panels). GAPDH was used as a loading control. Band intensities of GFAP were quantified (A and B, lower panel). Cell death was measured by monitoring LDH release (C, D). Neither miRNA inhibitors nor mimics induced cell death (C, D). Data are means±SEMs of three samples (*p<0.05; **p<0.01). Data shown are representative of at least three independent experiments.
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