Fig. 4. miR-326, miR-330, and miR-3099 bound to the GFAP 3'-UTR and regulated GFAP expression. (A) Schematic diagram of potential binding sequence (seed sequence) of each miRNA in the 3'-UTR of GFAP and mutagenesis scheme are shown. (B) Luciferase activity was measured 48 hours after transfecting HEK293T cells with pGL3-luciferase vectors (control, WT 3'-UTR of GFAP, mutant 3'-UTR of GFAP), pRL-Renilla reporter vectors, and each miRNA mimic (10 nM) using a Dual-Luciferase Reporter Assay System (right panel, H). The pRL-Renilla reporter was used to normalize for transfection efficiency. Data are means±SEMs of three samples (*p<0.05 vs. control; **p<0.01 vs. control). Data shown are representative of at least three independent experiments.
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