Fig. 2. The measurement of the β-galactosidase activity in N2A cells under high glucose condition and agmatine treatment. (A, B) The activity of β-galactosidase in experimental N2A cells was measured by β-galactosidase staining kit. The β-galactosidase positive cells (blue) were markedly increased in the glucose treatment group. Agmatine treatment were reduced the β-galactosidase positive cells in spite of the high glucose condition. Data were expressed as mean±S.E.M, and each experiment included 3 repeats per condition. Differences were considered significant at *p<0.05, **p<0.001. Glucose: glucose 200 mM treatment group, Agm: agmatine 100 µM treatment group, Agm+ Glucose: agmatine 100 µM and glucose 200 mM treatment group, senescence associated β-galactosidase (β-Gal): blue.
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