Fig. 1. Right-side whisker trimming in WT and AC5 KO mice changed the behavioral preference for AC5 KO pellets in opposite directions. (A, B) Diagram showing three WT-food vs . three KO-food pellets presented to each mouse (A; top panels) and food pellets remaining on the next day (A; bottom panels). The reduced sizes of food pellets on the bottom panels indicate that WT randomly ingested all six pellets, whereas AC5 KO mice preferentially consumed one AC5 KO-food pellet among 6 pellets (B). (C-E) Photographs showing a mouse with normal whiskers (left) and whiskers cut to the fur level (right). Whisker trimming in WT (D) and AC5 KO mice (E) reversed the behavioral preference for AC5 KO pellets over WT pellets. (F-I) AC5 KO mice with whiskers cut on the right side lost their behavioral preference for AC5 KO pellets (F, G), while WT mice with whiskers cut on the right side gained a behavioral preference for AC5 KO pellets (H and I). (J, K) siRNA-mediated inhibition of AC5 on the left side (J) or the right side (K) in AC5 heterozygous mice (AC5+/-) transiently induced a behavioral preference for AC5 KO pellets. Naive AC5+/- mice showed WT-like behaviors in this test. Data are presented as the mean±SEM (n=7-11). * and **denote the difference between the indicated groups at p<0.05 and p<0.01, respectively (Student's t-test, two-way ANOVA or two-way repeated-measures ANOVA, and post-hoc test).
© Exp Neurobiol
