Fig. 1. A diagram of optical recording of intrinsic signal (ORIS) imaging in the MCAO model. (A) The middle cerebral artery occlusion (MCAO) was performed for 60 min or 90 min and then reperfused. Behavior tests such as the walking evaluation (a), the beam balance test (b), and the sensory response test were conducted. (B) Illustration of ORIS imaging. The areas expected to react to the electrical stimulation were denoted as pale-red color (M1, motor cortex; S1HL, hindlimb somatosensory cortex; S1FL, forelimb somatosensory cortex; S1BF, barrel field somatosensory cortex). (C) The experimental scheme depicting ORIS imaging for 300 s and electrical stimulation (red arrow head) for 10 s is shown.
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