Fig. 4. T474 phosphorylation recruits the FAK phosphatase, SHP2. (A) FLAG-WT-FAK or T474E-FAK were overexpressed and immunoprecipitated using FLAG antibodies. The levels of SHP1, SHP2, PTPD1, and PTP-PEST bound to WT- or T474E-FAK were analyzed using antibodies specific for each protein. (B) Cells transfected with FLAG-WT-FAK or FLAG-T474E-FAK were treated with NSC-87877, an inhibitor of SHP2, and the levels of pY397 were determined by Western blot analysis.
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