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Fig. 1. Relative mRNA expression levels of SOCs in astrocytes. (a) Schematic diagram of three putative models for astrocytic SOCE components. ER Ca2+ depletion leads to Stim1 oligomerization and forms 1) Stim-Orai complex, 2) Stim-Orai/TrpC complex, or 3) Stim-TrpC complex. (b) Reproduced data from mRNA transcriptome database (Cahoy et al. (2008), and Zhang et al, (2014)), showing expression levels of Orai1, Orai2, Orai3, and TrpC1 in cultured astrocytes, p7 S100β+ astrocytes, p17 S100β+ astrocytes, and p1 Aldh1l1+ astrocytes. (c) The experimental scheme of measuring mRNA levels in primary cultured 7 DIV astrocytes by quantitative real-time PCR. (d) Relative mRNA expression of Orai1(100), Orai2 (31.25), Orai3 (49.75), TrpC1 (23.12), and “Housekeeping gene” GAPDH (Glyceraldehyde 3-phosphate dehydrogenase). Orai1 expression level was arbitrary set as 100 and GAPDH was measured as a control of gene expression (3956.79).
Exp Neurobiol 2017;26:42~54
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