Fig. 1. Locomotion defects by reducing the excitability of dopaminergic (DA) neurons in Drosophila larval brain. (A) A diagram to illustrate the UAS×Gal4 binary system for the expression of EKO in DA neurons. DA-specific driver TH-Gal4 encodes a transcription factor protein (Gal4P), which binds to upstream activating sequence (UAS) and then leads to EKO expression in DA neurons. (bottom) A representative image of a third instar Drosophila larva. (B) Examples of the larval crawling path for WT and TH-EKO larvae (four examples per each strain). Each path was recorded for 30 sec. Scale bar=3 cm. (C) Locomotion speed (mm/min) for various fly lines: WT and TH-Gal4 for control, TH-A53T for a PD gene α-Syn (TH-A53T), and TH-EKO. Number (n) of larvae tested in parenthesis. Mean±SEM. ***p<0.001 (one-way ANOVA and Dunnett's multiple comparison test).
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