Fig. 2. TH expression is decreased in mice with HFD-induced obesity. (A) TH immunoreactivity (red) was detected in the SN region of mice fed a NCD or HFD for 20 weeks. Upper panel, low magnification (×100) image; lower panel, higher magnification (×600) image. (B) The number of TH-positive neurons in the SN was counted and graphed (n=10 slides per condition, n=6/group). Sections were incubated at 4℃ with chicken anti-TH antibody (1:300), and fluorescence was visualized by confocal microscopy. (C) Immunohistochemical detection of TH in the striatum. Brain slices were incubated with a rabbit anti-TH antibody (1:300). After incubation with secondary antibody, diaminobenzidine staining was performed. (D) The TH-positive area was measured using ImageJ software. (E) The intensity of TH immunostaining in the NAc region was measured using ImageJ. Scale bars: 50 µm. *p<0.05, **p<0.01.
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