Fig. 1. Two- and three-dimensional co-culture of neurons and astrocytes. (A) Representative images of primary mouse neuron-astrocyte co-culture immunostained with anti-GFAP and anti-TuJ1 antibodies at DIV 10 for 2D and at DIV 5 for 3D. Nuclei are stained with Hoechst. Scale bars: 50 µm. (B) Three dimensional (3D) reconstruction of astrocyte images of (A). Scale bars: 20 µm.
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