Fig. 3. GABA content was increased in reactive astrocytes from 3D culture and hippocampal brain slice. (A) Immunostaining of neuron-astrocyte 3D co-culture with anti-GFAP, anti-GABA antibodies and Hoechst. (B) Quantification of GABA immunoreactivity in control and adenovirus-infected astrocytes. *p<0.05, unpaired student's t-test. (C) Immunostaining of adenovirus-injected hippocampal brain slice with anti-GFAP, anti-GABA antibodies and Hoechst. (D) Quantification of GABA immunoreactivity in control and adenovirus-infected conditions. ***p<0.001, unpaired student's t-test. (E) Schematic for virus injection into DG hippocampus. DG, dentate gyrus; ML, molecular layer.
© Exp Neurobiol
