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Fig. 7. Changes in TLR5 and MyD88 after postconditioning. (A) There was no difference in the amount of TLR5 between tMCAO and postconditioning groups. Compared with after tMCAO, MyD88 was slightly increased after postconditioning at 1 and 4 h reperfusion. MyD88 was captured by IP with an anti-MyD88 antibody in the total fraction. Results were detected by immunoblotting (IB) using antibodies to TLR5, p-Akt, and Akt. Coimmunoprecipitation analysis for TLR5/p-Akt showed an increase in the postconditioning group compared with the tMCAO group. Input lysates showed little difference between groups. Data are mean±SE. n=5 (#p<0.05, ##p<0.01, and ###p<0.001 compared with normoxia group). (B) Changes in TLR5 and MyD88 after OGD were in accordance with findings in vivo. Postconditioning increased the interaction of these proteins. Data are mean±SE. n=4 (#p<0.05 and ###p<0.001 compared with normoxia group; *p<0.05 compared with the OGD group).
Exp Neurobiol 2017;26:213~226
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