Fig. 4. Combining mutations with the V220W construct. HEK293 cells expressing mutated constructs were imaged under whole-cell voltage clamp conditions. The dark line represents the average of at least 4 cells. The shaded area is the standard error of the mean. Optical responses for V220W and R217Q/V220W are shown for two sets of command voltage pulses. A strong hyperpolizing pulse on the left was done to observe the maximal fluorescent signal present. Smaller hyperpolarization of the plasma membrane representing physiological ranges of neuronal inhibition are shown on the right.
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