| Constructs (V220X) | ∆F/Fmax (200 mV) | Weighted τ on | Weighted τ off |
|---|---|---|---|
| CC1 (WT) | 15±1.1 | 35±15.2 | 48±11.8 |
| V220G | 7±0.5 | 21±4.8 | 9±0.6 |
| V220A | 9±1.8 | 23±7.1 | 21±4.3 |
| V220L | 16±0.6 | 37±0.1 | 22±2.0 |
| V220I | 10±0.9 | >100 | >100 |
| V220M | 2±0.3 | ||
| V220C | 8±0.3 | 26±2.0 | 25±3.7 |
| V220P | 7±0.5 | 32±7.9 | 69±46.2 |
| V220F | 14±0.5 | 14±1.5 | 10±1.2 |
| V220W | 9±0.4 | 10±0.6 | 6±0.2* |
| V220Y | 8±0.7 | 9±0.4* | 6±0.9 |
| V220Q | 1±0.1 | ||
| V220N | 1±0.1 | ||
| V220S | 5±0.9 | 45±12.3 | 10±1.2 |
| V220T | 11±0.5 | 28±2.6 | 20±2.3 |
| V220H | 2±0.4 | ||
| V220D | 10±1.0 | 15±3.2 | 94±8.6 |
| V220E | 12±1.0 | 23±3.6 | 25±1.9 |
| V220R | 3±0.2 | 59±10.0 | 12±0.6 |
| V220K | 2±0.2 |
Constructs list the substitution to the V220 position. CC1 (WT) is V220. The ΔF/Fmax is the signal size for a 200 mV depolarization of the plasma membrane. The weighted on and off time constants are described in materials and methods. The time constants depicted with an asterisk exhibit single exponential fits. All other time constants were better fit with a double exponential function.