Fig. 5. Anti-inflammatory effects of silibinin on KA treatment in the hippocampus. (A) Representative coronal sections of the hippocampal CA1 region following Iba1 immunostaining 2 days after KA treatment in the hippocampus. Scale bars, 500 µm and 50 µm, respectively. (B, C) Western blot analysis of TNF-α and IL-1β expression in the hippocampus 2 days after KA treatment. The densities of TNF-α and IL-1β bands was normalized to the β-actin bands for each sample. *p<0.001 vs. PBS alone, #p<0.001 and ##p<0.05 vs. KA alone (one-way ANOVA and Tukey's post-hoc analysis; n=4, each experimental group). All values are expressed as the mean±SEM.
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