Fig. 3. Inhibition of HIF1α with chrysin induced cell death and suppressed the expressions of glycolysis-regulating genes in GBMs. (A~D) Cell viability levels of GBMs (U251, GBM28 and GBM37) and NSC10 in response to different doses (10, 50, or 100 µM) of chrysin or vehicle under normoxia (20% O2) and hypoxia (1% O2). Cells were treated with indicated doses of chrysin for 48 hours. Cell viability levels were measured with the MTT assay. (E~I) Representative images of RT-PCR (E) and quantified expression levels (F~I) of HIF1α, PDK1, PDK3, and GLUT1 in GBM cells treated with chrysin under normoxic (20% O2) and hypoxic (1% O2) conditions. Cells were treated with 100 µM of chrysin for 48 hours. Data are presented as the means±S.E.M (n=4 repeats in each group). β-actin was used as an internal control. *, **, and *** denote difference between control and indicated point at p<0.05, p<0.01, and p<0.001, respectively. #, ##, and ### denote difference between normoxic and hypoxic groups at p<0.05, p<0.01, and p<0.001, respectively (Student's t test, one-way ANOVA followed by post hoc test).
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