Fig. 3. Phenotypic changes in C. elegans models during polyQ transmission. (A, B) Nerve degeneration. Axonal processes from the URA motor neurons with DsRed fluorescence were analyzed for nerve degeneration. Worms with nerve fragmentation (A) and axonal blebs (B) in the URA motor neurons were quantified at days 8 and 13. Twenty worms for each line were analyzed; n=3, *p<0.05, ns: not significant. (C) Pharyngeal pumping rates at days 8 and 13. Twenty worms for each line were analyzed; n=3, **p<0.01, ***p<0.001. (D) Life-span analyses. The survival rates of all the lines in respective transgenic groups are plotted. One hundred worms for each line were analyzed. (E) Mean life span. One hundred worms in each line were analyzed; n=3, **p<0.01, ***p<0.001.
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