Fig. 6. Two GABAergic markers were absent in DACs of the rabbit retina. (A) A triple-labeled confocal image obtained at the border between the INL and IPL in a whole-mount preparation processed for TH (green), CR (blue), and GABA (red) immunoreactivity. A TH-labeled DAC with dendritic varicosities surrounding CR-labeled AII somata can be observed. The DAC soma (asterisk) and varicosities (arrowheads) do not demonstrate GABA immunoreactivity. (B) A double-labeled confocal image obtained at the stratum 1 of the IPL from a whole-mount preparation processed for TH (red) and GAD (green). Neither the DAC soma (asterisk) or dendritic varicosities (arrowheads) exhibit GAD immunoreactivity. Scale bars=20 µm. DACs: Dopaminergic amacrine cells; TH: tyrosine hydroxylase; INL: inner nuclear layer; IPL: inner plexiform layer; CR: calretinin; GAD: glutamic acid decarboxylase.
© Exp Neurobiol
