Fig. 6. Enhancement of zymosan phagocytosis by OPNpt20 in BV2 cells. BV2 cells were incubated with rhOPN (B; 1 µg/ml), OPNpt20 (C; 1 µg/ml), OPNpt20-Db (D; 1 µg/ml), or CytB (E; 1 µg/ml) for 1 hr in the presence of FITC-labeled zymosan particles and phagocytosis of zymosan particles was monitored every 5 minutes by live cell imaging analyzer. Percentages of internalized zymosan particles (F) and numbers of zymosan particles per cell (G) at 6 hrs after zymosan stimulation were quantified by counting 10 photographic fields. Results are presented as means±SEMs. *p<0.05, **p<0.01 versus zymosan-treated controls. #p<0.05 versus OPNpt20-treated control. Scale bars represent 50 µm.
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