Experimental Neurobiology

Table 3Summary of microbiota composition characterized in the present study and comparison with that identified from fecal samples in recent studies

	Present	Published
	APP/PS1^a) (serum)	APP/PS1^b) (fecal)	3xTg (fecal)
	APP/PS1^a) (serum)	(Harach et al., 2017)	(Bonfili et al., 2017)
<Microbial diversity>	WT vs. Tg	WT vs. Tg	WT vs. Tg
Alpha diversity	↑	↑	=

<Microbiome>		WT vs. Tg			WT vs. Tg	WT vs. Tg
Phylum	Genus	Fold change		Z-score	WT vs. Tg	WT vs. Tg
Actinobacteria			−1.48	−0.59
Actinobacteria	Corynebacterium	↓	−3.71	−1.45
Amatimonadetes			−9.95	−0.64
Bacteroidetes			−2.06	−0.73	↑
	Odoribacter	↑	<<	<<		↑
	Prevotella		2.35	0.61		↑
	f__Paraprevotellaceae;g__		n/s	n/s		↑
	f__Rikenellaceae;g__		1.05	0.08	↑
	f__S24-7;g__	↓	−3.49	−1.02	↑
	o__Bacteroidales;f__;g__	↑	<<	<<		↑
Cyanobacteria			−1.98	−0.56		↓
Cyanobacteria	o__YS2;f__;g__	↑	4.04	1.36		↓
Fibrobacteres		↑	<<	<<
Firmicutes		↑	1.66	3.45
	Aerococcus	↑	630.03	434.21
	Allobaculum		−1.01	−0.004	↓
	Anaerostipes		−172.96	−0.7		↓
	Blautia	↑	11.12	5.27
	Dorea	↑	<<	<<		↑
	Enterococcus		−4.97	−0.47		↑
	Jeotgalicoccus	↑	10.22	5.51
	Lactobacillus	↓	−1.78	−1.87
	Roseburia		1.09	0.06		↓
	Ruminococcus		1.22	0.25		↑
	SMB53		−2.56	−0.31		↑
	Streptococcus		1.12	0.16		↑
	Turicibacter		−1.18	−0.07		↑
	f__Christensenellaceae;g__		−3.59	−0.57		↑
	f__[Mogibacteriaceae];g__	↑	<<	<<		↑
	f__Peptostreptococcaceae;g__	↑	15.52	6.49		↑
	f__Ruminococcaceae;g__	↑	2.49	2.52
	o__Clostridiales; f__;g__	↑	1.51	1.06
Fusobacteria			−2.95	−0.46
Gemmatimonadetes			−260.4	−0.45
Proteobacteria		↓	−1.47	−1.56
	Escherichia/Shigella		−2.11	−0.27
	Flexispira		−2.22	−0.42		↑
	Pseudomonas	↑	1.48	1.58
	f__Desulfovibrionaceae; Other		n/s	n/s		↑
	f__Desulfovibrionaceae;g__	↑	<<	<<		↓
	f__Helicobacteraceae;g__		−2.02	−0.23		↓
	o__RF32;f__;g__		n/s	n/s		↓
Tenericutes		↓	−3.28	−1.1	↑	↑
	Anaeroplasma	↑	<<	<<		↓
	Mycoplasma		n/s	n/s		↓
	o__RF39;f__;g__	↓	−3.59	−1.14		↓
TM7			−1.46	−0.55
TM7	f__F16;g__		−1.62	−0.66		↓
[Thermi]		↑	<<	<<
Verrucomicrobia			1.29	0.38
Verrucomicrobia	Akkermansia		1.29	0.38	↓
Unassigned;other			1.48	0.39		↓
Unassigned;other	Unassigned;other		1.48	0.39		↓

The microbiomes for which the percent composition increased or decreased by more than one z score in Tg-APP/PS1 are marked by ↑ and ↓, respectively. « and » denote infinite increases and decreases, respectively. The increases and decreases of the indicated microbiota in the previous studies are also marked by ↑ and ↓, respectively.

The z-score is expressed as X − µ/σ, where µ=mean; X=individual score; σ=standard deviation (SD). ^a)APPswe/PSEN1dE9; ^b)APPswe/PSEN1-L166P.

Exp Neurobiol 2017;26:369~379 https://doi.org/10.5607/en.2017.26.6.369