Fig. 4. Changes in retinal organization after injection of substance P-saporin (SP-SAP) into the developing rat retina. Photomicrographs taken from vertical sections from control (A, C, E) and injected (B, D, F) retinas of postnatal day (PD) 14 rats immunostained for SP receptor (A, B), glycine transporter 1 (GLYT-1; C, D), and recoverin (E, F). (A) SP receptor-immunoreactive cells are seen in the proximal part of the inner nuclear layer (INL). (B) The distinct type of SP receptor-immunoreactive cells that are normally found at PD 6 to PD 12, remain at this age and a relatively continuous transient intermediate plexiform layer (TIPL) is also clearly seen. (C) GLYT-1-immunoreactive amacrine cells are seen in the proximal part of the INL. (D) Several GLYT-1-immunoreactive amacrine cells are found in the proximal part of the INL and two ectopically located GLYT-1-immunoreactive amacrine cells (arrows) of which soma are located in the middle part of the INL and processes are within the TIPL, are also found. (E) Recoverin-immunoreactive bipolar cells located in the distal part of the INL and their axon terminals ramified in the IPL are clearly seen. (F) Several recoverin-immunoreactive bipolar cells are found in the distal part of the INL and one ectopic recoverin-immunoreactive bipolar cell (arrow) located in the proximal part of the INL is clearly seen. In addition, some axons of recoverin-immunoreactive bipolar cells (arrowheads) ramify and terminate in the TIPL within the INL. Scale bar=50 µm.
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