Fig. 4. Dopaminergic signaling pathways are impaired in Ewsr1 KO (−/−) mice at 3 weeks of age. (A) qPCR analysis showed that Th mRNA was significantly lower in the striatum of Ewsr1 KO (−/−) mice (n=4) compared to WT mice (n=4) while Ddc (dopamine decarboxylase) and DARPP-32 mRNAs were not changed noticeably. (B) Western blot analysis showed that protein levels of TH and DARPP-32 were significantly decreased in Ewsr1 KO (−/−) mice (n=4) compared to WT mice (n=4). (C) The immunoreactivity of TH and DARPP-32 was markedly decreased in the striatum and the substantia nigra of Ewsr1 KO (−/−) mice, whereas the immunoreactivity of p-DARPP-32 (Thr75) was highly increased. (d) Densitometry analysis confirmed that the immunoreactivity of TH and DARPP-32 was significantly reduced in the striatum and substantia nigra of Ewsr1 KO (−/−) mice while the immunoreactivity of p-DARPP-32 (Thr75) was increased. Scale bar: 100 µm (black); 20 µm (red). WT (n=3); KO (−/−) (n=3). Data are presented as the mean±SEM. *p<0.05, **p<0.001, ***p<0.0001, unpaired Student's t test.
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