Fig. 3. Effect of aucubin on the differentiation into neurons producing GABA. Aucubin facilitates GABAergic differentiation of NPCs cultured primarily from the dorsal forebrain of pax6-GFP transgenic mice expressing recombinant GFP under the control of pax6 promoter. The cell survival of pax6-expressing late NPCs also increased in differentiation conditions. (A) Fluorescence images of NPCs in differentiating conditions with aucubin treatment (0, 0.01, 0.1, 1 and 10 µM). NPCs were stained with anti-GFP (green) or anti-GABA antibodies (red). Nuclei were counterstained with DAPI (blue). (B) Numbers of GFP-positive NPCs and (C) percentages of GABA-producing cells in differentiation medium; n=5 for each group. *p<0.05 by one-way ANOVA. The scale bar indicates 20 µm.
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