Fig. 1. Experimental setup and histological verification of the stimulation site. (A) Experimental setup for ICSS. A mouse was positioned in a custom-made stimulation chamber with free access to a wheel. When the mouse turned the wheel by 1/4 of a rotation, a trigger signal from a rotation sensor was sent to both a pulse stimulator and an analog-to-digital (A/D) converter. The pulse stimulator gave the electrical stimulation to the MFB following a protocol arranged in the stimulator. Prefrontal EEG and hippocampal LFP data were acquired by an analog amplifier, digitalized at 500 Hz and recorded by a computer. (B) Histological verification of the tip of the stimulating electrode. CM, central medial thalamic nucleus; ic, internal capsule; mt, mammillothalamic tract; mfb, medial forebrain bundle; 3v, third ventricle.
© Exp Neurobiol