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Fig. 1. Schematic diagram of behavioral procedures. Eight weeks old male C57Bl/6 mice were used in 3 separate cohorts. SPS-CF was exposed by 5 days of electric shock (1 mA, 2 sec) combined with white noise (80 dB) and light illumination, followed by immobilization (2 hr), forced swim (20 min), and ether anesthesia in the 6th day. Then mice were left undisturbed for 7 days. In cohort1, contextual and cued freezing, alternation in the Y-maze, locomotion in the open field and anxiety levels in the elevated plus maze and novelty suppressed feeding test was measured. In cohort 2, spatial reference memory was measured in the Morris water maze. Then mice were sacrifice for tissue collection. In cohort 3, concentration-dependent effects of RP on contextual and cued fear was measured.
Exp Neurobiol 2018;27:200~209
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