Fig. 3. Effects of RP on SPS-CF stress-induced memory impairment of spatial memory. (A) Latency to find hidden platform in Morris water maze during 5 daily training sessions (n=9 for VeN and RPN, n=10 for VeS and RPS). Three-way ANOVA demonstrated a significant effect by daily repetition of training (F4,170=15.9, p<0.001), SPS-CF exposed stress (F1,170=9.2, p=0.003), and RP administration (F1,170=26.8, p<0.001). Only stress × RP administration showed a significant interaction (F1,170=4.9, p=0.028). Post-hoc multiple comparison showed a significant effect of RP on reducing the latency in SPS-CF (p<0.001). (B) Time spent in the target quadrant during the probe trial of Morris water maze test (n=38). Two-way ANOVA demonstrated a significant effect of SPS-CF exposure (F1,34=32.5, p<0.001), but not of RP administration (F1,34=2.9, p=0.097) and SPS-CF exposure × RP interaction (F1,34=0.4, p=0.53). (C) Working memory performance in the in the Y maze. Two-way ANOVA demonstrated no significant effect neither by SPS-CF exposure (F1,21=0.68, p=0.42), RP administration (F1,21=0.04, p=0.84) and SPS-CF × RP interaction (F1,21=1.7, p=0.21) in % alternation. (D) Spontaneous locomotion in the open field during 30 minutes. Two-way ANOVA demonstrated no significant effect either by SPS-CF stress (F1,21=3.75, p=0.11), RP administration (F1,21=0.6, p=0.45) and SPS-CF × RP interaction (F1,21=0.86, p=0.37) in the total distance traveled. All data represent mean±SEM. VeN, vehicle fed, non-stressed; RPN, RP 0.1 mg/kg fed, non-stressed; VeS, vehicle fed, SPS-CF exposed; RPS, RP 0.1 mg/kg fed, SPS-CF exposed. ***p<0.001; non-stressed vs SPS-CF exposed. ###p<0.001; VeS vs RPS.
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