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Fig. 6. HPLC analysis of RP key components. The HPLC analysis was performed on an Agilent 1100 series liquid chromatograph with diode array detector interfaced with an Agilent Chem. Station for the data analysis. The column was a 4.6 mm ID×250 mm Zorbax Eclipse Plus C18 column (5 µm pore size; Agilent, CA) with a Security GuardTM guard cartridge (3.0×4.0 mm, Phenomenex, CA). The mobile phase was composed of 0.1% phosphoric acid in water (a) and acetonitrile (b) using the following linear gradient program: 0~20 min, 10~40% B; 20~30 min, 40~50% B. Chromatography was carried out in gradient mode using a flow rate of 1.2 ml/min at 25℃ and was detected at various UV wavelength of individual standards and polygalae radix sample. Four standards (Norharmane, TMCA, DISS, Tenuifolin) were prepared at 16.8, 23.8, 75.5, 680.4 µg/ml, respectively. Polygalae radix extract powder was dissolved in DMSO at 20 mg/ml. (A) The retention time of Norharmane was 8.613 minute measured at 254 nm. (B) The retention time of DISS was 13.15 minute measured at 330 nm. (C) The retention time of TMCA was 18.68 minutes measured at 310 nm. (D) The retention time of tenuifolin was 21.38 minute measured at 202 nm.
Exp Neurobiol 2018;27:200~209
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