Fig. 2. Disease state-specific alteration of activity-dependent BDNF secretion from cortical axons. (A) Average fluorescence changes with time (ΔFt/F0) for BDNF-pH puncta of cortical axons in wild-type (Emx1-Cre/+, black) or Q140 (Emx1-Cre Q140/+, red) during the presymptomatic period (10 weeks old) or symptomatic period (1-year-old). Mean±s.e.m. HFS (black bar) was given to trigger activity-dependent BDNF secretion. (B) Bar graphs depict average ΔFt/F0 (±s. e. m.) during 100~200 s after stimulation for all recorded BDNF-pH puncta. Numbers of puncta from at least 3 slices of 2 mice: presymptomatic wild-type (WT), 56; presymptomatic Q140 (Q140/+), 82; symptomatic wild-type (WT), 62; symptomatic Q140 (Q140/+), 155. *p<0,05, ***p<0.001, unpaired t-test. (C) Bar graphs depict average fractional changes of individual BDNF-pH puncta (putative BDNF-containing vesicles, mean±s.e.m.). WT vs. Q140: *p<0.05, **p<0.01, unpaired t-test.
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