Fig. 5. Morin inhibits apoptotic signaling molecules increased by KA injection. Morin was orally administered to mice 1 day before the KA injection, then administered for 2 more days. (A, B) Double-immunofluorescence staining for NeuN (red) and c-caspase-3 (green), and NeuN (red) and c-PARP-1 (green) in the hippocampal CA1 regions. Scale bar, 20 µm, 5 µm. (C, D) Western blot analysis of c-caspase-3, caspase-3, c-PARP-1, and PARP-1 expression in the hippocampus. The density of c-caspase-3, caspase-3, c-PARP-1, and PARP-1 bands was normalized to the β-actin band for each sample. *p<0.05 and **p<0.01 vs. CON, #p<0.05 vs. KA alone (one-way ANOVA and Tukey's post-hoc analysis; n=4, each experimental group). All values are expressed as the mean±SEM.
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