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Fig. 1. In vitro properties of Igf1r-heterozygous NSCs. (A) Representative images of neurospheres derived from NSCs wild-type (+/+) and heterozygous (+/−) for Igf1r gene. Since Igf1r-heterozygous line was crossed with DsRed.T3 strain, all neurospheres emit intense red fluorescence. Scale bars indicate 50 µm. Quantification graph comparing mean diameters of +/+ and +/− neuropsheres. N=4 independent cultures for each group. (B) Representative bright-field images at a lower magnification. Scale bars indicate 100 µm. Quantification graph comparing the number of +/+ and +/− neuropsheres. N=4 independent cultures for each group. (C, D) In vitro survival assays. NSCs were exposed to either 3-morpholinosydnonimine (SIN-1, 400 µM) (C) or H2O2 (1mM) (D) for 48 h with or without IGF-1 (20 ng/ml). The percentage of viable cells in the treatment groups was expressed as the percent value compared with that of control condition (without any treatment). N=4 independent cultures per group. ** and *** represent p<0.01 and p<0.001 compared to control condition, and ### represents p<0.001 comparing SIN-1 (C) or H2O2 (D) exposure groups with or without IGF-1 treatment by one-way ANOVA followed by Tukey's post hoc analysis at each time point.
Exp Neurobiol 2018;27:489~507
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