Fig. 3. Survival of NSC grafts in the injured spinal cord. (A, B) Representative images of longitudinal spinal cord sections at 4 (A) or 8 weeks (B) after injury. Spinal cord sections were immunostained with anti-RFP (red) and anti-glial fibrillary acidic protein (GFAP; green) antibodies to visualize the distribution of the transplanted NSCs in relation to the lesions. Scale bars indicate 1 mm. (C) Representative integrated drawing of stereologically counted RFP-positive NSCs at the 8-week time point. RFP-positive NSCs from 5 longitudinal sections 300 µm apart from each other were integrated in one image. The structural outlines of the spinal cord tissue in the integrated images were obtained from the most middle section in each animal. Scale bar indicates 1 mm. (D) Quantification graphs of the number of surviving NSCs at 4 and 8 weeks after injury. N=5 and 9 animals at 4 and 8 weeks, respectively, for each group. * and ** represent p<0.05 and p<0.01, respectively, by two-way ANOVA followed by post hoc Bonferroni analysis.
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