Fig. 4. Phenotypic differentiation of NSCs after transplantation into the injured spinal cord. (A~D) Representative images of transplanted NSCs in the injured spinal cord from animals receiving Igf1r (+/+) NSC grafts with (C) or without (A) TMT, and from animals receiving Igf1r (+/−) NSC grafts with (D) or without (B) TMT. Transplanted cells were identified by immunoreactivity to red fluorescence protein (RFP). Neural cell phenotypes were determined by immunoreactivity (green) for neuronal marker DCX, mature oligodendrocyte marker APC-CC1, and astrocytic marker GFAP. RFP-positive NSCs (red) colocalized with neural cell markers are shown in yellow. Higher magnification and orthographic projection images of the boxed regions are shown at the bottom of each panel. All scale bars indicate 20 µm. (E) Quantification graphs of the number of differentiated NSCs at 8 weeks after injury. N=9 animals, for each group.
© Exp Neurobiol
