Fig. 6. In vitro migration ability of NSCs. (A) Representative images of the bottom side of the Boyden chamber membrane separating upper and lower chambers. The membrane was stained to visualize NSCs that migrated to the bottom side. Scale bar indicates 50 µm. (B) Representative still images acquired at the designated time points of migratory NSCs in the scratch assay. Live imaging was performed to monitor migrating NSCs to the scratch created at the center of the culture plate. Scale bar indicates 200 µm. (C) Quantification graph of the Boyden chamber assay. *, **, and *** represent p<0.05, p<0.01, and p<0.001, respectively, by one-way ANOVA followed by post hoc Tukey's analysis. N=4 independent assays for each group. (D) Quantification graph of the scratch assay. Statistical analysis was done with the percent area of cell occupancy data obtained at 12-h time intervals. *** represents p<0.001 by repeated measures two-way ANOVA followed by post hoc Bonferroni analysis. N=3 independent assays for each group.
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