Fig. 1. Differentiation of HCN cells into neurons, oligodendrocytes, and astrocytes. (A) A schematic timeline for differentiation experiments. (B) Undifferentiated and differentiated HCN cells were stained for Nestin, MAP2, RIP, and GFAP (green) with Hoechst 33342 (blue), and imaged by confocal microscopy. Scale bar, 25 µm. (C) Sox2 mRNA levels in undifferentiated HCN cells (Con) and HCN cells differentiated into neurons (Neuron), oligodendrocytes (Oligo), or astrocytes (Astro). (D) Changes in NeuN, GalC and Gfap mRNA levels after differentiation. *p<0.05, **p<0.01, and ***p<0.001. n≥3.
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