Fig. 2. Time course analyses of HCN cell differentiation into astrocytes. (A) A schematic timeline for astrogenesis experiments. D, day. (B) Images of HCN cells stained with nestin and GFAP antibodies at D0 and D4 during astrogenesis. Scale bar, 50 µm. (C) Astrocyte morphology examined with GFAP staining. Scale bar, 25 µm. (D) GFAP and Sox2 protein levels analyzed by Western blotting. (E) Gfap mRNA levels. (F) Tnc mRNA levels. (G) mRNA levels of other neural cell markers, NeuN and GalC . ns, not significant. **p<0.01, and ***p<0.001. n≥3.
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