Fig. 3. An increase in autophagy flux from early time points during astrogenesis. (A) Analysis of autophagy flux using mRFP-GFP-LC3 stable HCN cells. Scale bar, 15 µm. (B) After Baf.A1 treatment, autophagy flux was measured in mRFP-GFP-LC3 stable HCN cells at D2. Baf.A1 (20 nM) was added 1 h before harvesting. Scale bar, 15 µm. (C) Time course analyses of LC3-II by Western blotting. (D) Time course analyses of autophagy flux by Western blotting of LC3-II after Baf.A1 treatment. Baf.A1 (20 nM) was added 1 h before harvesting. (E) Increased autophagy flux at D2. Baf.A1 (20 nM) or PepA/E64d (10 µg/ml for each) was treated 1 h before harvesting. *p<0.05, **p<0.01, and ***p<0.001. n≥3.
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