Fig. 4. Impaired astrogenesis by the suppression of autophagy in HCN cells. (A) Verification of Atg7 knockdown by Western blotting analysis. (B, C) Decrease in GFAP protein (B) and mRNA (C) levels in Sh-Atg7 cells compared with Sh-Con cells. (D) Impaired astrocyte morphology in Sh-Atg7 cells compared with Sh-Con cells at D4. (E) Verification of LC3 knockdown by Western blotting analysis. (F) Decrease in Gfap mRNA level in Sh-LC3 cells. (G) Reduction of Gfap and Tnc mRNA levels by Baf.A1 treatment. Baf.A1 (0.5 nM) was added at D0 and D2 and half of media was changed at D1 and D3. Scale bar, 25 µm. *p<0.05, **p<0.01, and ***p<0.001. n≥5.
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