Fig. 5. Impaired astrogenesis in p62 knockout HCN cells. (A) Genetic deficiency of p62 abrogated an increase in GFAP expression in differentiated p62 knockout HCN cells (Sg-p62) in comparison with control cells (Sg-Con) at D4. (B) A dramatic decrease in Gfap mRNA expression level in Sg-p62 cells in comparison with Sg-Con cells at D4. (C) Impaired astrocyte morphology in Sg-p62 cells in comparison with Sg-Con cells at D4. Scale bar, 25 µm. (D) The absence of induction of cell death during astrogenesis. (E~G) Recovery of GFAP protein (E), mRNA (F) levels, and astrocyte morphology (G) by expression of GFP-p62, but not GFP-EV (empty vector) in Sg-p62 cells at D4. Scale bar, 25 µm. *p<0.05, **p<0.01, and ***p<0.001. n≥3.
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