Fig. 4. Overexpression of the human RAPGEF2-E1357K variant in Drosophila motor neurons impairs microtubule stability in axons and at NMJ terminals. (A~D) Confocal images of motor axons (A and B) and NMJ terminals (C and D) of abdominal segment 5 in third instar larvae doubly labeled with anti-acetylated α-tubulin (Ac-tub) or anti-Futsch and anti-HRP antibodies. Genotypes include D42-GAL4/+ (control), D42-GAL4/UASHA-RAPGEF2-WT(HA-RAPGEF2-WT), UAS-HA-RAPGEF2-E1357K/+; D42-GAL4/+ (HA-RAPGEF2-E1357K), and D42-GAL4/UAS-gef26RNAi(Gef26 KD). Scale bars, 5 µm. (E and F) Quantification of the ratios of mean Ac-tub to HRP fluorescence intensities in axons (E) and at NMJ terminals (F). (G and H) Quantification of the ratios of mean Futsch to HRP fluorescence intensities in axons (G) and at NMJ terminals (H). Data are presented as mean±SEM. All comparisons are made with the D42-GAL4/+ control (*p<0.001).
© Exp Neurobiol
