Fig. 2. Histological examination of the spinal cord and olfactory bulb of control and EAE-affected mice (G.3, paralytic stage). (A) No inflammatory cells were present in the spinal cord of control mice. (B) In EAE-affected mice, inflammatory cells (arrow) and perivascular cuffing (arrowhead) were detected in the spinal cord. (C) In control mice, there was no inflammatory response in the olfactory bulb. (D) Inflammatory cells were identified in the olfactory bulb of EAE-affected mice. Some Iba-1-positive microglia (E) and glial fibrillary acidic protein (GFAP)-positive astrocytes (G) were detected in the olfactory bulb of the control. Iba-1-positive microglia (F) and GFAP-positive astrocytes (H) were increased in the olfactory bulb of EAE-affected mice. (A~D) Hematoxylin and eosin staining. (E and F) Iba-1 immunostaining. (G and H) GFAP immunostaining. Counterstained with hematoxylin. ePL, external plexiform layer; GL, glomerulus layer; GrL, granular cell layer; ML, mitral cell layer; ONL, olfactory nerve layer. Arrowheads, perivascular cuffing; arrows, inflammatory cells. Scale bars=50 µm.
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