Fig. 2. Effects of miR-381 on PMP22 expression. (A) Schematic diagram of plasmid constructs used to show specificity of miR-381 in targeting the 3′UTR of PMP22 gene. Arrows show target sites of each miRNA. (B) Western blotting showing that miR-381 and miR-9 specifically target the 3′UTR of PMP22. RT4 cells were transfected with either control myc vector (Con), Myc-hPMP22, or Myc-hPMP22-3′UTR vector along with negative control miRNA (mock), miR-381, or miR-9. Quantitation of Western blotting results were displayed (bottom). The band intensity was determined by the Image J program (
https://imagej.nih.gov) then normalized to GAPDH. (C) Quantitative RT-PCR analysis showing that miR-381 specifically reduces mRNA level of
PMP22 flaked with 3′UTR in the same setting in RT4 cells.
*p<0.05;
**p<0.01.
