Fig. 3. Quantitation of mRNA and protein levels in sciatic nerves. (A) Quantitative real-time PCR analysis of miR-381 from sciatic nerves of C22 mice at 15 weeks post administration showing efficient delivery of LV-miR-381. (B and C) Quantitative real-time PCR analysis of human PMP22 (B) and mouse Pmp22 (C) showing that the mRNA level was decreased in LV-miR-381 treated C22 mice compared to that in control-miRNA injected C22 mice (mock). (D) Immunofluorescence with human specific PMP22 (green) showing significantly decreased expression level in LV-miR-381 treated C22 mice. Blue, Hoechst staining. *p<0.05.
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