Fig. 2. The KH3 domain of Nova-1 is responsible for 5-HT6R binding. (A) Schematic diagram of HA- and venus C-terminal (VC)-tagged full length and truncated Nova-1 constructs (top). GST-tagged CT of 5-HT6R (GST-6RCT) (bottom). (B) The expression of HA-VC-tagged full length or truncated Nova-1 constructs was identified by immunoblotting with anti-HA antibody. (C) GST pull-down assay showed that GST-6RCT interacted with all three different Nova-1 constructs. (D) Specific interaction between GST-6RCT and three different Nova-1 constructs was validated by co-immunoprecipitation. (E) Co-immunoprecipitation assay showed that endogenous 5-HT6R interacted with all three different Nova-1 constructs. (F) 5-HT6R CT interacted with GST-KH3 but not with GST-Nova-1 (239–419 aa).
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