Fig. 2. AMPK is involved in HO-1 induction by KMS99220. (A) BV2 cells were treated with various concentrations of KMS99220 for 6 h or 24 h, and RT-PCR and western blot analysis was performed against HO-1. For quantitation, values obtained from densitometry were normalized against respective loading controls (GAPDH or β-actin) and are expressed as induction fold of untreated control, as indicated above each panel. (B) BV2 cells were pretreated with Ara A or Compound C for 1 h, KMS99220 was added to the final concentration of 10 µM, and the cells were further cultured for 6 h. RT-PCR was performed against HO-1 using GAPDH as a loading control. For quantitation, values obtained from densitometry were normalized against GAPDH and are expressed as fold of KMS99220-treated control.
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