Fig. 1. Effect of MPTP on dopaminergic neuronal loss and neuroinflammation in the substantia nigra of cynomolgus monkeys. (A) TH protein expression level in the substantia nigra of saline- or MPTP-injected cynomolgus monkeys was determined by using western blotting. (B) Immunohistochemistry staining of TH-positive neurons in the cynomolgus monkey brain injected with saline or MPTP was performed using anti-TH antibody. The bottom panels show magnified images of the substantia nigra pars compacta (SNpc) region indicated by the black squares in the top panels; scale bars=10 mm. (C) Fluorescent imaging results of TH proteins were validated using anti-TH antibody in the substantia nigra of saline- or MPTP-injected cynomolgus monkeys; scale bars=100 µm. (D) GFAP and Iba-1 protein expression in the substantia nigra of saline- or MPTP-injected cynomolgus monkeys was confirmed using western blotting. (E) Expression of GFPA and Iba-1 proteins were identified in the SNpc region of saline- or MPTP-injected cynomolgus monkeys using immunohistochemistry; scale bars=2 µm. C1 and C2 indicate the saline-injected group, and C3 and C4 indicate the MPTP-injected group. The data are presented as mean values±SD (n=2). ***denotes p<0.001.
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