Fig. 3. Chronic immobilization stress (CIS) and glutamine supplementation induced expressional changes of GLT1 and SNATs in the medial prefrontal cortex. Representative mPFC immunohistochemistry images of (A) glutamate transporter GLT1 and (B~E) sodium-coupled neutral amino acid transporters (SNATs) merged with a cellular marker (GFAP and GS for astrocytes, NeuN and GLS2 for neurons). Scale bars: 25 µm. Arrowheads indicate double-positive cells and arrows indicate cellular marker single-positive cells. Double-positive cell numbers are presented with the images (n=12/group, cell number/0.15 mm2). *p<0.05, **p<0.01, ***p<0.001 (vs. CTL), #p<0.05, ##p<0.01, ###p<0.001 (vs. STR), one-way ANOVA with Newman-Keuls multiple comparison test as post-hoc analysis.
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