Fig. 1. TLR4 expression was significantly increased in the ipsilateral dorsal horn of rats with SNL-induced neuropathic pain. (A) To establish a rat model of neuropathic pain, rats sensitive to von Frey filaments (>10 g filaments) were subjected to lumbar 5 (L5) SNL. Then, the von Frey filament test was performed on days 3, 7, and 14 post-ligations. Data are presented as means ± SEM (one-way analysis of variance (ANOVA) with Dunnett's post hoc test, ***p<0.001 vs. BL of SNL Ipsi; n=11 per group). (B) On days 3, 7, and 14 after surgery, the L4–6 segments of the ipsilateral dorsal horn were processed and used for isolation of total RNA. After cDNA synthesis, the TLR4 mRNA level was determined by qPCR analysis. Sham operation was performed as a control. Data are presented as means±SEM (one-way ANOVA with Tukey's post hoc test, **p<0.01 vs. BL, ##p<0.01 and #p<0.05; n=4–5 per group). BL, baseline; Ipsi, Ipsilateral.
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